Key result
Antisense oligodeoxynucleotides complementary to the 5' end of TMV RNA inhibited in vitro translation of the genomic RNA, leaving 10-25% of the control signal at molar ratios ≥1:15.
Population
In vitro translation of tobacco mosaic virus (TMV) genomic RNA in a rabbit reticulocyte lysate
Comparison
Two different 'antisense' oligodeoxynucleotides… vs Control signal or oligodeoxynucleotides/RNA…
Design
Preclinical
Authors
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May inform nucleic acid antiviral development; leaves open in vivo efficacy and clinical translation.
Antisense oligodeoxynucleotides and their RNA analogues can directly interfere with ribosome function to selectively inhibit TMV RNA translation in vitro.
Crum et al. (1988) studied Tobacco mosaic virus (TMV) RNA translation. Antisense oligodeoxynucleotides and their RNA analogues vs. Control signal / sense oligodeoxynucleotides was evaluated on In vitro translation of the genomic RNA. Antisense oligodeoxynucleotides complementary to the 5' end of TMV RNA inhibited in vitro translation of the genomic RNA, leaving 10-25% of the control signal at molar ratios ≥1:15.
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