Key result
Radial immunodiffusion assay detects urinary myoglobin in ~98% of AMI patients versus zero controls.
Why the study?
Does radial immunodiffusion assay for myoglobinuria accurately detect acute myocardial infarction compared to standard serum enzymes?
Case-Control (n=100)
Does radial immunodiffusion assay for myoglobinuria accurately detect acute myocardial infarction compared to standard serum enzymes?
Myoglobinuria detected by radial immunodiffusion assay is a highly accurate and early diagnostic marker for acute myocardial infarction.
Answers are grounded in this paper and cite their sources.
Myoglobinuria assay may aid early AMI detection; hypothesis-generating for diagnostic superiority versus modern biomarkers in prospective studies.
The technique of radial immunodiffusion assay, in which the gel is impregnated with myoglobin antiserum, was used on urine samples from 60 patients with acute myocardial infarction, from 20 patients with chest pain due to ischemic heart disease without infarction, and from 20 normal subjects. In the group with an acute myocardial infarct, all but one patient showed significant levels of myoglobin (at or above 5 mg/ml) in urine samples taken within 48 hours after clinical onset of infarct. Urinary myoglobin to these levels never rose in the other two groups studied. Myoglobinuria appeared sooner, persisted longer, and was a much more accurate diagnostic test for infarction than serum levels of glutamic oxaloacetic transaminase, lactic dehydrogenase, or creatine phosphokinase. With these data, it will be possible to prepare a simple latex or agglutination test for myoglobinuria, to detect acute myocardial damage. (JAMA228:1251-1255, 1974)
No takes yet. Share an insight, caveat, or question.
H. J. Saranchak (1974) conducted a case-control in Acute myocardial infarction (n=100). Radial immunodiffusion assay for urinary myoglobin vs. Patients with ischemic chest pain without infarction and normal subjects was evaluated on Detection of significant levels of myoglobin (≥5 mg/ml) in urine. Radial immunodiffusion assay detected significant urinary myoglobin (≥5 mg/ml) in 98.3% of patients with acute myocardial infarction within 48 hours, compared to 0% in control groups.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: