What question did this study set out to answer?

Determine the functional significance of Arp2 and Arp3 nucleotide binding in Arp2/3 complex nucleation activity and cellular actin organization.

January 17, 2005The Journal of Cell BiologyOpen Access

Effects of Arp2 and Arp3 nucleotide-binding pocket mutations on Arp2/3 complex function

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Authors

AMAdam C. MartinMoscow Institute of Thermal Technology XXXiaoping XuBGI Group (China)IRIsabelle RouillerBiotechnology Institute

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Implication

Mutational analysis demonstrates critical roles for Arp2 and Arp3 nucleotide binding in yeast, indicating essential structural functions during endocytosis.

Key Points

Determine the functional significance of Arp2 and Arp3 nucleotide binding in Arp2/3 complex nucleation activity and cellular actin organization.
Engineered nucleotide-binding pocket mutations in Arp2 and Arp3 within Saccharomyces cerevisiae.
Assessed in vitro actin filament nucleation activity alongside in vivo actin dynamics and endocytic trafficking.
Examined structural conformational changes in complex containing an Arp2 suppressor mutant using electron microscopy.
ATP binding to both Arp2 and Arp3 was necessary for full in vitro nucleation activity, with nucleotide-bound Arp3 proving essential for cortical actin cytoskeleton architecture.
Defects in endocytosis did not track with reductions in in vitro nucleation activity, pointing to a structural rather than catalytic role during endocytic uptake.
An Arp2 suppressor mutant enhanced actin nucleation and adopted a conformation identical to wild-type complex activated by nucleation promoting factors.

Cite This Study

Martin et al. (2005) studied this question.

synapsesocial.com/papers/6a9c6dbe69a24bf63e7e6b83 https://doi.org/10.1083/jcb.200408177

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Implication

Mutational analysis demonstrates critical roles for Arp2 and Arp3 nucleotide binding in yeast, indicating essential structural functions during endocytosis.

Effects of Arp2 and Arp3 nucleotide-binding pocket mutations on Arp2/3 complex function

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Implication

Key Points

Cite This Study

Effects of Arp2 and Arp3 nucleotide-binding pocket mutations on Arp2/3 complex function

Discussion

Member takes

Implication

Key Points

Cite This Study

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