Key result
Tetraplex qPCR screening of 323 fish specimens identified NoV GI genomic RNA in a pool of seabass brains, with a 1-log difference in quantification between plasmid and RNA templates.
Why the study?
Increasing raw fish consumption has heightened the risk of foodborne viral infections from human enteric viruses such as norovirus and hepatitis A virus.
Observational (n=323)
The study demonstrates the presence of human norovirus in fish specimens, highlighting a potential risk of viral infection through raw fish consumption.
Human enteric viruses such as norovirus (NoV) and hepatitis A virus (HAV) are some of the most important causes of foodborne infections worldwide. Usually, infection via fish consumption is not a concern regarding these viruses, since fish are mainly consumed cooked. However, in the last years, raw fish consumption has become increasingly common, especially involving the use of seabass and gilthead seabream in dishes like sushi, sashimi, poke, and carpaccio. Therefore, the risk for viral infection via the consumption of raw fish has also increased. In this study, a virologic screening was performed in 323 fish specimens captured along the Portuguese coast using a tetraplex qPCR optimised for two templates (plasmid and in vitro transcribed RNA) to detect and quantify NoV GI, NoV GII and HAV genomes. A difference of approximately 1-log was found between the use of plasmid or in vitro transcribed RNA for molecular-based quantifications, showing an underestimation of genome copy-number equivalents using plasmid standard-based curves. Additionally, the presence of NoV genomic RNA in a pool of seabass brains was identified, which was shown to cluster with a major group of human norovirus sequences from genogroup I (GI.1) by phylogenetic analysis. None of the analysed fish revealed the presence of NoV GII or HAV. This result corroborates the hypothesis that enteric viruses circulate in seawater or that fish were contaminated during their transportation/handling, representing a potential risk to humans through raw or undercooked fish consumption.
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Filipa-Silva et al. (2021) conducted an observational in Human enteric viruses (NoV and HAV) contamination (n=323). Tetraplex qPCR screening was evaluated on Detection and quantification of NoV GI, NoV GII, and HAV genomes. Tetraplex qPCR screening of 323 fish specimens identified NoV GI genomic RNA in a pool of seabass brains, with a 1-log difference in quantification between plasmid and RNA templates.
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