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A technique for the cultivation of amphibian leukocytes, using heparinized blood and Bacto-Phytohemagglutinin, is described. The karyotype of Necturus m. maculosus, obtained from leukocyte mitoses, is presented. The diploid chromosome number of this salamander is 38. These chromosomes are classified into three groups, according to length and position of the centromere. The chromosome number presented here corrects the erroneous count of King (1912).
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Seto et al. (1964) studied this question.
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