Metabolic Behavior and Chlortetracycline Production by Streptomyces aureofaciens in Liquid Culture

The present paper deals with an experimental study of the metabolic behavior of Streptomyces aureofaciens' grown in laboratory fermentors. EXPERIMENTAL METHODSStandard fermentations were performed as follows: A spore suspension was inoculated into 3 L of the following medium: sucrose, 3 per cent; corn steep liquor, 1 per cent; molasses, 0.1 per cent; NaCl, 0.05 per cent; and CaCO3, 0.4 per cent.The fermentations were carried out at 27 C in 5-L fermentors (Bartholo- mew, Karow and Sfat, 1950).After 36 hours, 100 ml of the culture fluid was trans- ferred into three liters of the fermentation standard medium (U.S. Patent 1952): sucrose, 3 per cent; corn steep, 1 per cent; CaCO3, 1 per cent; (NH4)2 SO4, 0.2