Diversity and Potential Cross-Species Transmission of Rotavirus A in Wild Animals in Yunnan, China

Figures

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  Figure 1. The phylogenetic relationship between the VP7 (a) and VP4 (b) and closely related strains and genotype reference strains screened in this study was investigated (red font). (a) Based on the VP7 gene, MEGA-X was constructed using the maximum likelihood method (ML), and model (GTR+G), and bootstrap analysis was performed 1000 times. (b) Based on the VP4 gene, MEGA-X was constructed using the maximum likelihood method (ML) and model (GTR+G), and bootstrap analysis was performed 1000 times.

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  Figure 2. The phylogenetic relationship between the structural genes VP1 (a), VP2 gene (b), VP3 gene (c) and VP6 gene (d) of rotavirus A in wild animals screened in this study with closely related strains and genotype reference strains (red font). (a) Based on the VP1 gene, MEGA-X was constructed using the maximum likelihood method (ML), and model (GTR+G+I), and bootstrap analysis was performed 1000 times. (b) Based on the VP2 gene, MEGA-X was constructed using the maximum likelihood method (ML), and model (GTR+G), and bootstrap analysis was performed 1000 times. (c) Based on the VP3 gene, MEGA-X was constructed using the maximum likelihood method (ML), and model (GTR+G+I), and bootstrap analysis was performed 1000 times. (d) Based on the VP6 gene, MEGA-X was constructed using the maximum likelihood method (ML), and model (T92+G), and bootstrap analysis was performed 1000 times.

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  Figure 3. The phylogenetic relationship between the non-structural genes NSP1 (a), NSP2 gene (b), NSP3 gene (c), NSP4 gene (d) and NSP5 gene (e) of rotavirus A in wild animals screened in this study and closely related strains and genotype reference strains (red font). (a) Based on the NSP1 gene, MEGA-X was constructed using the maximum likelihood method (ML), and model (GTR+G), and bootstrap analysis was performed 1000 times. Based on the NSP2 gene, MEGA-X was constructed using the maximum likelihood method (ML), and model (TN93+G), and bootstrap analysis was performed 1000 times. (c) Based on the NSP3 gene, MEGA-X was constructed using the maximum likelihood method (ML), and model (GTR+G), and bootstrap analysis was performed 1000 times. (d) Based on the NSP4 gene, MEGA-X was constructed using the maximum likelihood method (ML) and and model (HKY+G) for 1000 bootstrap analyses. Based on the NSP5 gene, MEGA-X was constructed using the maximum likelihood method (ML), and model (T92+G), and bootstrap analysis was performed 1000 times.