What question did this study set out to answer?

To develop an improved, rapid, and sensitive in vivo photofootprinting technique to map protein-DNA interactions in intact yeast cells.

January 1, 1989Nucleic Acids ResearchOpen Access

An lmproved method for photofootprinting yeast genesin vivousing Taq polymerase

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Authors

JAJeffrey D. AxelrodStanford University JMJohn MajorsUniversity of Southern California

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Implication

Methodological study demonstrates sensitive in vivo genomic photofootprinting using Taq polymerase in yeast, highlighting improved detection of transcription factor binding.

Key Points

To develop an improved, rapid, and sensitive in vivo photofootprinting technique to map protein-DNA interactions in intact yeast cells.
Irradiated intact yeast cells with ultraviolet light to introduce DNA photoproducts across the genome.
Detected and mapped photoproducts at nucleotide resolution through iterative rounds of primer annealing and extension with thermostable Taq polymerase.
Provided a more rapid, sensitive, and reproducible detection of photoproducts compared to earlier chemical photofootprinting procedures.
Successfully mapped GAL4 protein binding to its upstream activating sequence sites within the GAL1-10 promoter directly in vivo.
Demonstrated broader utility of the primer extension assay for mapping DNA strand breaks and direct genomic sequencing in yeast.

Cite This Study

Axelrod et al. (1989) studied this question.

synapsesocial.com/papers/6a9d6a6d8abc1d8b83a4bfec https://doi.org/10.1093/nar/17.1.171

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Implication

Methodological study demonstrates sensitive in vivo genomic photofootprinting using Taq polymerase in yeast, highlighting improved detection of transcription factor binding.

An lmproved method for photofootprinting yeast genesin vivousing Taq polymerase

Discussion

Member takes

Implication

Key Points

Cite This Study

An lmproved method for photofootprinting yeast genesin vivousing Taq polymerase

Discussion

Member takes

Implication

Key Points

Cite This Study

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