What question did this study set out to answer?

To provide a comprehensive, detailed protocol for assessing load-dependent and load-independent cardiac hemodynamics in a rat model at baseline and 28 days post-myocardial infarction.

January 1, 2016International Journal of Clinical and Experimental Medical SciencesOpen Access

Assessment of Experimental Permanent Coronary Artery Ligation Using Echocardiography and Invasive Real-Time Pressure-Volume (PV): A Practical Tips to Evaluate Rat Hemodynamics

Q: What is the clinical evidence from this study?

Population: Myocardial ischemia and infarct injury. Intervention: Permanent coronary artery ligation vs. Baseline/Control. Primary outcome: Hemodynamics (including LVEDV, LVESV, and EF) (p=<0.001).

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Key result

Permanent coronary artery ligation in rats significantly altered hemodynamics at 28 days, including increased LVEDV (319 vs 215 µl; P<0.001) and decreased ejection fraction (50% vs 76%; P<0.001).

Key Points

To provide a comprehensive, detailed protocol for assessing load-dependent and load-independent cardiac hemodynamics in a rat model at baseline and 28 days post-myocardial infarction.
Induced myocardial infarction and ischemic remodeling in rats via surgical permanent coronary artery ligation.
Evaluated cardiac mechanics and chamber remodeling at baseline and 28 days post-ligation using 2D M-mode echocardiography and invasive pressure-volume (PV) loop catheterization.
Ligation produced severe chamber dilation with increased LVEDV (319±73 vs. 215±79 µl; P<0.001) and LVESV (157±39 vs. 57±28 µl; P<0.001), accompanied by reduced ejection fraction (50±8.5 vs. 76±4.9%; P<0.001).
Systolic contractility deteriorated with significant decreases in dpdtmax (5786±1443 vs. 9609±4126 mmHg; P<0.01), end-systolic pressure (91.5±12 vs. 108.2±13 mmHg; P<0.001), and cardiac output (49±12 vs. 68±3.5 ml/min; P<0.05).
Diastolic dysfunction worsened by day 28, marked by elevated LVEDP (9.1±2.9 vs. 5.18±2.5; P<0.01), prolonged filling times, and an increased heart weight-to-body weight ratio reflecting myocardial hypertrophy (P<0.001).

Structured PICO

Population

Rat model of permanent coronary artery ligation assessed at baseline and 28 days post-myocardial ischemia.

Intervention

Permanent coronary artery ligation

Comparator

Baseline hemodynamics

Outcome

Load-dependent and independent hemodynamics assessed by 2D echocardiography and invasive pressure-volume (PV) catheterizationsurrogate

Main Result

p-value: p=<0.001

Rat permanent coronary artery ligation induces significant systolic and diastolic dysfunction at 28 days, providing a reliable preclinical model for heart failure.

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

No clinical implications yet; leaves open translation of this rat ligation model to human heart failure therapies.

Cite This Study

Filip Konečný (2016) studied Myocardial ischemia and infarct injury. Permanent coronary artery ligation vs. Baseline/Control was evaluated on Hemodynamics (including LVEDV, LVESV, and EF) (p=<0.001). Permanent coronary artery ligation in rats significantly altered hemodynamics at 28 days, including increased LVEDV (319 vs 215 µl; P<0.001) and decreased ejection fraction (50% vs 76%; P<0.001).

synapsesocial.com/papers/6a9d6c24671a7532065786c8 https://doi.org/10.11648/j.ijcems.20160203.12

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Assessment of Experimental Permanent Coronary Artery Ligation Using Echocardiography and Invasive Real-Time Pressure-Volume (PV): A Practical Tips to Evaluate Rat Hemodynamics

Key Points

Structured PICO

Main Result

Discussion

Implication

Cite This Study

Assessment of Experimental Permanent Coronary Artery Ligation Using Echocardiography and Invasive Real-Time Pressure-Volume (PV): A Practical Tips to Evaluate Rat Hemodynamics

Key Points

Structured PICO

Main Result

Discussion

Implication

Cite This Study

Also Consider

Also Consider