Key result
Staphylococcal phage lysate stimulated lymphoproliferative responses in both T and B cell subpopulations in vitro, with 95% positive responses in lymphocyte stimulation assays.
p-value: p=<0.01
SPL is a useful reagent for in vitro study of cell-mediated reactivity, stimulating both T and B lymphocytes.
SPL may aid in vitro lymphocyte studies; leaves open any clinical translation pending human validation.
The cell-mediated reactivity of normal individuals to staphylococcal phage lysate (SPL) were tested in vitro in the lymphocyte stimulation (LS) and leukocyte migration inhibition (LMI) assays. There were 95% positive responses in LS (stimulation ratio larger than or equal to 3 with p less than 0.01) and 67% positive responses in LMI (migration index less than or equal to 0.80). Enriched subpopulations of T and B lymphocytes were prepared with rosette formation and density gradient centrifugation. SPL stimulated lymphoproliferative responses in both T and B cell subpopulations whereas phytohemagglutinin (PHA) stimulated only the T cell subpopulation. Cord blood leukocytes were tested in the LS assay and 41% gave positive responses to SPL, 81% to PHA, and 17% with SLO. SPL appears to be a useful reagent for the in vitro study of cell-mediated reactivity, and may provide somewhat different information from that obtained with other mitogens or antigens.
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Dean et al. (1975) studied Normal individuals. Staphylococcal phage lysate (SPL) vs. Phytohemagglutinin (PHA) and SLO was evaluated on Cell-mediated reactivity (lymphocyte stimulation and leukocyte migration inhibition) (p=<0.01). Staphylococcal phage lysate stimulated lymphoproliferative responses in both T and B cell subpopulations in vitro, with 95% positive responses in lymphocyte stimulation assays.
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