Key result
The in-house recombinant nucleocapsid protein-based ELISA demonstrated 100% sensitivity and specificity for detecting infectious bronchitis virus antibodies in chicken flocks.
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An in-house recombinant nucleocapsid protein-based ELISA is a highly sensitive and specific alternative to commercial kits for detecting chicken infectious bronchitis virus antibodies.
Infectious bronchitis (IB) is one of the devastating chicken viral diseases that causes significant financial loses to chicken industry worldwide. In this study, highly purified recombinant nucleocapsid protein (rNP) of IB virus (IBV) was used to develop an in-house enzyme linked immunosorbent assay (iELISA) for the detection of IB. The iELISA was optimized to achieve maximum sensitivity and specificity. All tested broiler chicken farms were positive for IBV antibody in a commercial ELISA kit (cELISA) and the optimized iELISA. Thirty three (33) specific pathogen free (SPF) chickens and 20 broiler chickens held in a restricted environment tested negative for IBV antibody in both ELISA. The above results suggest that the iELISA is a sensitive and specific test for the detection of antibody to IBV in chicken flocks.
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Gholamreza Haqshenas (2013) studied Chicken infectious bronchitis (n=280). In-house recombinant nucleocapsid protein-based ELISA (iELISA) vs. Commercial ELISA kit (cELISA) was evaluated on Detection of infectious bronchitis virus (IBV) antibodies (Sensitivity and Specificity). The in-house recombinant nucleocapsid protein-based ELISA demonstrated 100% sensitivity and specificity for detecting infectious bronchitis virus antibodies in chicken flocks.
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