Why the study?
Avian infectious bronchitis is highly contagious, and reliable immune monitoring after vaccination is critical for poultry.
Does a novel S2-derived peptide-based ELISA accurately detect broadly cross-reactive antibodies against infectious bronchitis virus in poultry?
Does a novel S2-derived peptide-based ELISA accurately detect broadly cross-reactive antibodies against infectious bronchitis virus in poultry?
Answers are grounded in this paper and cite their sources.
A novel S2-derived peptide-based ELISA provides a rapid and reliable serological surveillance tool for monitoring infectious bronchitis virus infection or vaccination in poultry.
Avian infectious bronchitis (IB) is a highly contagious disease caused by infectious bronchitis virus (IBV). Vaccination is an effective approach for controlling IBV. Therefore, reliable immune monitoring for IB is critical for poultry. In this study, a novel peptide derived from S2 protein was used to develop an enzyme-linked immunosorbent assay (ELISA) for the detection of broadly cross-reactive antibodies against IBV. The peptide-based ELISA (pELISA) showed good specificity and sensitivity in detecting IBV antibodies against different serotypes. A semilogarithmic regression method for determining IBV antibody titers was also established. Antibody titers detected by pELISA and calculated with this equation were statistically similar to those evaluated by indirect fluorescence assay (IFA). Moreover, the comparison analysis showed a 96.07% compatibility between the pELISA and IDEXX ELISA. All these data demonstrate that the pELISA generated here can be as a rapid and reliable serological surveillance tool for monitoring IBV infection or vaccination.
No takes yet. Share an insight, caveat, or question.
Xie et al. (2023) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: