Key result
A liquid phase blocking ELISA for detecting infectious bronchitis virus antibodies showed high correlation with the serum neutralization test (r2 = 0.8926), with 88% sensitivity and 100% specificity.
Why the study?
Does LPB-ELISA accurately detect antibodies against infectious bronchitis virus in chicken sera compared to SNT?
Does LPB-ELISA accurately detect antibodies against infectious bronchitis virus in chicken sera compared to SNT?
Effect estimate: r2 = 0.8926
LPB-ELISA is a rapid, sensitive, and specific test for detecting antibodies against infectious bronchitis virus in chickens.
May support LPB-ELISA as a practical alternative to SNT in poultry serology; leaves open routine adoption pending prospective validation.
A liquid phase blocking ELISA (LPB-ELISA) was developed for the detection and measurement of antibodies against infectious bronchitis virus (IBV). The purified and nonpurified virus used as antigen, the capture and detector antibodies, and the chicken hyperimmune sera were prepared and standardized for this purpose. A total of 156 sera from vaccinated and 100 from specific pathogen-free chickens with no recorded contact with the virus were tested. The respective serum titers obtained in the serum neutralization test (SNT) were compared with those obtained in the LPB-ELISA. There was a high correlation (r2 = 0.8926) between the two tests. The LPB-ELISA represents a single test suitable for the rapid detection of antibodies against bronchitis virus in chicken sera, with good sensitivity (88%), specificity (100%) and agreement (95.31%).
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Cardoso et al. (1999) studied Infectious bronchitis virus (IBV) antibodies (n=256). Liquid phase blocking ELISA (LPB-ELISA) vs. Serum neutralization test (SNT) was evaluated on Correlation between LPB-ELISA and SNT, sensitivity, specificity, and agreement (r2 = 0.8926). A liquid phase blocking ELISA for detecting infectious bronchitis virus antibodies showed high correlation with the serum neutralization test (r2 = 0.8926), with 88% sensitivity and 100% specificity.
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