Key result
Physiologic concentrations of tissue factor pathway inhibitor (< 8 nmol/L) do not inhibit formed prothrombinase, whereas higher concentrations (~100 nmol/L) effectively block its activity.
Population
In vitro prothrombin activation assays using purified components of the prothrombinase complex
Design
Preclinical
Authors
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Physiologic TFPI may not inhibit prothrombinase in vivo; leaves open whether higher concentrations or analogs warrant thrombosis trials.
Contrary to amidolytic assays, prothrombinase is resistant to inhibition by physiological concentrations of TFPI in the presence of prothrombin, requiring much higher concentrations for effective blockade.
Mast et al. (1996) studied this question. Tissue factor pathway inhibitor (TFPI) was evaluated on Thrombin generation / prothrombinase inhibition. Physiologic concentrations of tissue factor pathway inhibitor (< 8 nmol/L) do not inhibit formed prothrombinase, whereas higher concentrations (~100 nmol/L) effectively block its activity.
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