Key result
Expression of bovine Mx1 protein in BHK-21 cells reduced foot-and-mouth disease virus yields by 90% and viral VP1 mRNA levels by 60% compared to control cells.
Effect estimate: 90% reduction in viral yields
p-value: p=<0.05
Bovine Mx1 protein expression inhibits the replication of foot-and-mouth disease virus in vitro, suggesting a potential antiviral mechanism.
Mx proteins belonging to the dynamin superfamily of large GTPases inhibit replication of a wide range of RNA viruses. In this study, we examined whether bovine Mx1 protein could interfere with the replication of foot-and-mouth disease virus (FMDV). For this purpose we established cloned BHK-21 cells expressing bovine Mx1 protein (BM1 cells) and infected them with FMDV serotype O. Cloned BHK-21 cells expressing neomycin resistance instead of Mx1 protein (BH1 cells) and original BHK-21 cells served as negative controls. The results showed that the expression of bovine Mx1 protein reduced viral yields by 90% and levels of viral VP1 mRNA by 60%. These findings correlated with a significant reduction of viral antigen detectable in infected cells by immunofluorescent assay. These results demonstrate that bovine Mx1 protein interferes with the replication of FMDV.
No takes yet. Share an insight, caveat, or question.
Cai et al. (2013) studied Foot-and-mouth disease virus (FMDV) infection (in vitro). Expression of bovine Mx1 protein vs. BHK-21 cells expressing neomycin resistance (BH1 cells) and original BHK-21 cells was evaluated on Viral yields and levels of viral VP1 mRNA (90% reduction in viral yields, p=<0.05). Expression of bovine Mx1 protein in BHK-21 cells reduced foot-and-mouth disease virus yields by 90% and viral VP1 mRNA levels by 60% compared to control cells.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: