What is the clinical evidence from this study?

Study design: Other. Population: Acute gastroenteritis (human astrovirus infection). Intervention: RT-PCR with AC230/AC1' primer set. Primary outcome: Nucleotide and amino acid sequence identity, and detection of HAstV types.

May 1, 2000Journal of Medical Virology

Genomic characterization of human astrovirus type 6 Katano virus and the establishment of a rapid and effective reverse transcription-polymerase chain reaction to detect all serotypes of human astrovirus

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Key result

A newly established RT-PCR system using the AC230/AC1' primer set based on the ORF2 region of human astrovirus type 6 allows for rapid and highly sensitive detection of HAstV serotypes.

Population

Human astrovirus type 6 strains, specifically Katano virus K23 and K24 from a 1991 outbreak of acute…

Design

Preclinical

Authors

NSNaomi SakonOsaka Prefectural Institute of Public Health KYKenji YamazakiHealth Outcomes Solutions (United States)EUEtsuko UTAGAWANational Institute of Infectious Diseases

Discussion

Member takes

Implication

Offers new HAstV research tool; leaves open human validation before clinical or epidemiologic use.

Structured PICO

Population

Human astrovirus type 6 (HAstV T6) strains, specifically Katano virus K23 and K24 from a 1991 outbreak of acute gastroenteritis in Katano City, Osaka, Japan

Exposure

Reverse transcription-polymerase chain reaction (RT-PCR) detection system using the AC230/AC1' primer set designed from the 5'-terminal end region of ORF2

Outcome

Genomic characterization (sequencing of the open reading frame 2 [ORF2] region) and establishment of a detection system for HAstV typessurrogate

A rapid and highly sensitive RT-PCR system was established for detecting human astrovirus serotypes, which may aid in the epidemiologic characterization of astrovirus infections.

Cite This Study

Sakon et al. (2000) studied Acute gastroenteritis (human astrovirus infection). RT-PCR with AC230/AC1' primer set was evaluated on Nucleotide and amino acid sequence identity, and detection of HAstV types. A newly established RT-PCR system using the AC230/AC1' primer set based on the ORF2 region of human astrovirus type 6 allows for rapid and highly sensitive detection of HAstV serotypes.

synapsesocial.com/papers/6a9dab29fa7e3bbd20d9aa79 https://doi.org/10.1002/(sici)1096-9071(200005)61:1<125::aid-jmv20>3.0.co;2-b

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Genomic characterization of human astrovirus type 6 Katano virus and the establishment of a rapid and effective reverse transcription-polymerase chain reaction to detect all serotypes of human astrovirus

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Genomic characterization of human astrovirus type 6 Katano virus and the establishment of a rapid and effective reverse transcription-polymerase chain reaction to detect all serotypes of human astrovirus

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider