Key result
A newly developed RT-PCR method for detecting astroviruses demonstrated about 10 times higher sensitivity compared to enzyme immunoassay with monoclonal antibody.
The developed RT-PCR method provides higher sensitivity than EIA-MAb for detecting astroviruses in clinical stool samples.
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May improve astrovirus detection in stool samples; leaves open prospective clinical validation.
We developed a reverse transcription and polymerase chain reaction (RT-PCR) method for detecting astrovirus serotypes 1, 2, 3, 5, 6 and 7 (but not serotype 4). Furthermore, we developed the specific primers for detecting serotypes 1 and 2, the most predominant serotypes in the world. Sensitivity of the first PCR with serotype common primers was about 10 times higher than that of enzyme immunoassay with monoclonal antibody (EIA-MAb). Sensitivity of the second PCR with the serotype-specific primers was even higher. The RT-PCR method was useful for detecting astroviruses from clinical samples, especially serotypes 1 and 2.
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Saito et al. (1995) studied Astrovirus infection. Reverse transcription and polymerase chain reaction (RT-PCR) vs. Enzyme immunoassay with monoclonal antibody (EIA-MAb) was evaluated on Sensitivity for detecting astroviruses. A newly developed RT-PCR method for detecting astroviruses demonstrated about 10 times higher sensitivity compared to enzyme immunoassay with monoclonal antibody.
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