Key result
Multiplex RT-PCR and monoplex RT-PCR with degenerate primers detected astroviruses in 59.4% of samples, which was 25% higher than detection by electron microscopy (34.4%).
Absolute Event Rate: 59.4% vs 34.4%
Newly developed RT-PCR assays demonstrated higher sensitivity and a wider detection spectrum for Turkey astroviruses compared to traditional electron microscopy.
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RT-PCR may improve astrovirus detection in turkeys; leaves open effects on disease management or outcomes.
Tang et al. (2005) studied Turkey astrovirus (poult enteritis). AC-ELISA and RT-PCR assays vs. Electron microscopy (EM) was evaluated on Detection rate of astroviruses. Multiplex RT-PCR and monoplex RT-PCR with degenerate primers detected astroviruses in 59.4% of samples, which was 25% higher than detection by electron microscopy (34.4%).
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