Key result
Multiplex RRT-PCR demonstrated sensitivity varying by target between 150 gene copies for TAstV-2 alone and 2200 gene copies for TCV, with cloacal swabs showing similar sensitivity to intestinal samples.
Population
Poults inoculated at 1 day of age with turkey coronavirus, turkey astrovirus type 2, and avian reoviruses…
Comparison
Multiplex Real-Time Reverse… vs Virus isolation and intestinal samples
Design
Preclinical
Follow-up
21 days after inoculation
Authors
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Cloacal swabs may enable sensitive multiplex detection of turkey enteritis viruses; leaves open prospective field validation before clinical adoption.
A multiplex RRT-PCR assay was successfully developed for the sensitive and simultaneous detection of three viruses associated with Poult Enteritis Complex in turkeys.
Spackman et al. (2005) studied Poult enteritis complex (PEC). Multiplex Real-Time Reverse Transcription-Polymerase Chain Reaction (RRT-PCR) vs. Virus isolation / Intestinal samples was evaluated on Assay sensitivity and virus detection. Multiplex RRT-PCR demonstrated sensitivity varying by target between 150 gene copies for TAstV-2 alone and 2200 gene copies for TCV, with cloacal swabs showing similar sensitivity to intestinal samples.
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