International Journal of Current Research in Biology and Medicine

In the process of drug discovery, the current strategy upon which the scientist relies on the alternate source is from natural origin.Due to prevailing side effect on regular usage of allopathic medicine people of developing countries started migrating towards the traditional healing therapy like Siddha.Siddha system of medicine pioneering in emphasizes the biological activity of the various phytocomponents with respect to the etiology and pathophysiology of various dread full diseases emerging in humans and animals.It is evident that there are some metallic preparations used in Siddha as potency of acting as analgesics, anti-microbial, immune modulators, Hepato, neuro and nephron protectant.Siddha system of medicine is one of the oldest traditional healing therapies known to the mankind since several centuries.It has numerous novel formulations which benefit the society for various ailments.As per the AYUSH guideline Siddha system of medicine has standard protocol starting from collection of raw material, purification, formulation and biological evaluation.The main aim of the present investigation is to carry out the physicochemical evaluation of the drug Manosilai and to make the comparative analysis of the Manosiali before and after purification.The results obtained from physicochemical analysis clearly reveals that the Loss on drying at 105 o C of Manosilai before and after purification was found to be 0.4481 and 0.2953 %w/w.Total Ash value found to be 4.088 and 3.892 %w/w similarly acid insoluble value of Manosilai before and after was found to be 0.0809 and 0 %w/w.Water Soluble Extractive value was found to be 1.028 and 0.9300 %w/w.Similarly, alcohol soluble extractive value was 0.2795 and 0.5559 % w/w.Results of microbial analysis reveals the absence of pathogenic organism including bacteria and fungi.Further with respect to the toxins there is an absence of aflatoxin B1, B2, G1 and G2was observed in both before and after purification.It is essential for a formulation to be free from pesticide residues in order to ensure that the Manosilai was subjected to GC MS and LC MS analysis and the results clearly indicates that pesticide level is below the limit of quantification.It was concluded from the results of the present investigation that the drug Manosilai complies with the standards described by the AYUSH.