Key result
Recombinant ASFV protein p54-specific ELISA showed almost perfect agreement with the gold standard immunoblotting test for detecting ASFV antibodies (kappa 0.95; 95% CI 0.90-0.99).
Why the study?
Do recombinant ASFV protein-based ELISAs provide reliable serodiagnosis of African swine fever compared to gold standard tests in porcine sera?
Population
Porcine field sera including European, West African, and East African pigs, plus warthog sera.
Comparison
Four recombinant ASF virus protein-based… vs Gold standard immunoblotting and OIE-approved…
Design
Preclinical
Authors
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Supports p54 ELISA validation for ASF serodiagnosis; leaves open clinical translation pending prospective porcine studies.
Cross-Sectional (n=574)
Do recombinant ASFV protein-based ELISAs provide reliable serodiagnosis of African swine fever compared to gold standard tests in porcine sera?
Effect estimate: kappa 0.95 (95% CI 0.90 to 0.99)
Recombinant ASFV proteins p54, pB602L, and pK205R provide sensitive and specific targets for detecting African swine fever antibodies in European and West African pigs and warthogs.
Gallardo et al. (2009) conducted a cross-sectional in African swine fever (n=574). Recombinant ASFV protein-based ELISAs (pK205R, pB602L, p104R, and p54) vs. Gold standard immunoblotting and OIE-approved ELISA was evaluated on Agreement with gold standard test (kappa value) (kappa 0.95, 95% CI 0.90 to 0.99). Recombinant ASFV protein p54-specific ELISA showed almost perfect agreement with the gold standard immunoblotting test for detecting ASFV antibodies (kappa 0.95; 95% CI 0.90-0.99).
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