Key result
The m35 recombinant protein-based indirect ELISA demonstrated high diagnostic accuracy for African swine fever virus, with an area under the curve of 0.9738, a sensitivity of 93.1%, and a specificity of 96.55%.
Why the study?
African swine fever causes severe disease and major economic losses, and verified diagnostic antigens such as p30 and p54 can aid in diagnosis.
Does a novel multi-epitope recombinant protein m35 provide accurate diagnosis of African swine fever in swine serum samples?
Does a novel multi-epitope recombinant protein m35 provide accurate diagnosis of African swine fever in swine serum samples?
Effect estimate: AUC 0.9738 (95% CI 0.9336 to 1.014)
A novel multi-epitope recombinant protein m35 demonstrates high diagnostic accuracy for African swine fever, offering a potential new tool for disease detection.
Supports m35 ELISA development for ASF in swine; leaves open field validation before diagnostic adoption.
Background: African swine fever(ASF) is an acute, severe and highly fatal infectious disease of pigs. The disease spreads rapidly, causing huge economic losses to the pig industry in infected areas. The structural proteins p30 and p54 in African swine fever virus(ASFV) have been verified as diagnostic antigens.Methods: In this study, we constructed a novel multi-epitope fusion antigen gene based on P30 and P54 proteins, induced expression in a prokaryotic expression system and analyzed the reactivity of the recombinant fusion protein. The purified recombinant protein m35 was used as the coating antigen to establish an indirect enzyme-linked immunosorbent assay (ELISA) detection method for ASFV. 116 serum samples and positive sera of other swine diseases were detected by indirect ELISA.Results: Our results indicate that the m35 gene fragment with a length of 558bp was successfully constructed. SDS-PAGE and Western Blotting analysis showed that the protein had a band at 22kDa, proving its good reactogenicity. ROC analysis was performed to validate the assay, the area under the ROC curve is 0.9738 (95% confidence interval, 0.9336 to 1.014), and does not cross-react with other swine diseases.Conclusion: Our results show that its sensitivity and specificity were highly accurate. It is feasible to use this recombinant protein as a diagnostic antigen to distinguish ASFV infection.
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Gao et al. (2020) studied African swine fever (n=116). m35 recombinant protein-based indirect ELISA vs. Western blot (reference standard) was evaluated on Diagnostic accuracy (Area under the ROC curve) (AUC 0.9738, 95% CI 0.9336 to 1.014). The m35 recombinant protein-based indirect ELISA demonstrated high diagnostic accuracy for African swine fever virus, with an area under the curve of 0.9738, a sensitivity of 93.1%, and a specificity of 96.55%.
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