Screening and identification of the dominant antigens of the African swine fever virus

Figure 1. Reaction of ASFV recombinant proteins with antibodies in pig sera. ELISAs based on purified ASFV proteins were constructed and used to test five clinical ASFV-positive sera from farm pigs (C group, blue dot), 10 ASFV-positive pig sera experimentally infected with low-virulence ASFV (E group, green dot) and five ASFV-negative pig sera (N group, orange). The data of each group are presented as means and SD and were tested for significance using Student's t-test. * indicates a significant difference between the C group and N group (*, P < 0.05; **, P < 0.01; and ***, P < 0.001). # indicates a significant difference between the E group and N group (#, P < 0.05; ##, P < 0.01; and ###, P < 0.001).

Figure 2. Development of ASFV-specific antibodies in pigs infected with low-virulence ASFV. ELISA methods based on 15 ASFV recombinant proteins including p30 (A), p54 (B), p34 (C), p22 (D), pB475L (E), pE184L (F), pA104R (G), pI73R-pB169L (H), pC129R (I), pK145R (J), pE199L (K), pE120R (L), p12-p17-p10 (M), pK205R (N), and pCP312R (O) were used to test the sera of four pigs (the number 25, 26, 28, and 29) at 6, 10, 15, and 21 dpi with low-virulence ASFV. A total of five ASFV-negative pig sera served as negative controls. If S/N≥3 (cutoff value), the sample was positive for antibodies against ASFV protein.