Key result
A double-antigen sandwich ELISA based on recombinant p30 protein was successfully developed for African swine fever virus antibody detection, exhibiting excellent specificity and sensitivity.
Why the study?
African swine fever poses a major threat to the swine industry, but effective vaccines and diagnostic methods to prevent and control the disease are currently lacking.
Does a double-antigen sandwich ELISA based on p30 protein effectively detect antibodies against African Swine Fever Virus?
Does a double-antigen sandwich ELISA based on p30 protein effectively detect antibodies against African Swine Fever Virus?
A newly developed double-antigen sandwich ELISA using recombinant p30 protein provides an effective, sensitive, and specific method for detecting African Swine Fever Virus antibodies.
May enable p30-based ASF serological diagnostics; leaves open field validation before clinical adoption.
African swine fever (ASF), the highly lethal swine infectious disease caused by the African swine fever virus (ASFV), is a great threat to the swine industry. There is no effective vaccine or diagnostic method to prevent and control this disease currently. The p30 protein of ASFV is an important target for serological diagnosis, expressed in the early stage of viral replication and has high immunogenicity and sequence conservatism. Here, the CP204L gene was cloned into the expression vector pET-30a (+), and the soluble p30 protein was successfully expressed in the E. coli prokaryotic expression system and then labeled with horseradish peroxidase (HRP) to be the enzyme-labeled antigen. Using the purified recombinant p30 protein, a double-antigen sandwich ELISA for ASFV antibody detection was developed. This method exhibits excellent specificity, sensitivity and reproducibility in clinical sample detection with lower cost and shorter production cycles. Taken together, this study provides technical support for antibody detection for ASFV.
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Wang et al. (2022) studied African swine fever. Double-antigen sandwich ELISA based on p30 protein was evaluated on Antibody detection (specificity, sensitivity, and reproducibility). A double-antigen sandwich ELISA based on recombinant p30 protein was successfully developed for African swine fever virus antibody detection, exhibiting excellent specificity and sensitivity.
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