Key result
A dual-antigen indirect ELISA using CP204L and B602L was successfully established for detecting ASFV antibodies, demonstrating no cross-reactivity and good performance compared to commercial kits.
Why the study?
African swine fever lacks an effective vaccine or diagnostic method to prevent and control the disease, highlighting the need for early detection.
A novel dual-antigen indirect ELISA provides a low-cost, sensitive, and specific method for the serological diagnosis of African swine fever.
Supports ASFV ELISA development in swine; leaves open diagnostic adoption pending prospective validation.
African swine fever (ASF) is an acute, virulent, and highly fatal infectious disease caused by the African swine fever virus (ASFV). There is no effective vaccine or diagnostic method to prevent and control this disease currently, which highlights the significance of ASF early detection. In this study, we chose an early antigen and a late-expressed antigen to co-detect the target antibody, which not only helps in early detection but also improves accuracy and sensitivity. CP204L and B602L were successfully expressed as soluble proteins in an Escherichia coli vector system. By optimizing various conditions, a dual-antigen indirect ELISA for ASFV antibodies was established. The assay was non-cross-reactive with antibodies against the porcine reproductive and respiratory syndrome virus, classical swine fever virus, porcine circovirus type 2, and pseudorabies virus. The maximum serum dilution for detection of ASFV-positive sera was 1:1600. The intra-batch reproducibility coefficient of variation was <5% and the inter-batch reproducibility coefficient of variation was <10%. Compared with commercial kits, the dual-antigen indirect ELISA had good detection performance. In conclusion, we established a detection method with low cost, streamlined production process, and fewer instruments. It provides a new method for the serological diagnosis of ASF.
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Zhou et al. (2023) studied African swine fever. Dual-antigen indirect ELISA based on p30 (CP204L) and pB602L vs. Commercial kits was evaluated on Detection performance, cross-reactivity, and reproducibility. A dual-antigen indirect ELISA using CP204L and B602L was successfully established for detecting ASFV antibodies, demonstrating no cross-reactivity and good performance compared to commercial kits.
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