Key result
Prohibiting phosphorylation with AMP-PNP or ATP omission prevented the rundown of calcium-activated chloride currents in rabbit pulmonary artery myocytes, maintaining currents at ~100% vs ~20% in controls.
Absolute Event Rate: 100% vs 20%
Phosphorylation status of the Ca2+-activated Cl- channel complex dramatically influences current generation through voltage-dependent steps in pulmonary arterial smooth muscle cells.
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Prevents Cl- current rundown in rabbit myocytes; leaves open relevance to human pulmonary vascular tone or disease.
Angermann et al. (2006) studied this question. Pipette solutions prohibiting phosphorylation (AMP-PNP or ATP omitted) vs. Control conditions with ATP and 500 nM Ca2+ was evaluated on Calcium-dependent Cl- currents (I(ClCa)) rundown. Prohibiting phosphorylation with AMP-PNP or ATP omission prevented the rundown of calcium-activated chloride currents in rabbit pulmonary artery myocytes, maintaining currents at ~100% vs ~20% in controls.
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