Key result
Deletion of 285L or K145R from the ASFV genome did not significantly affect viral spread and productive growth in wild boar lung cells or primary macrophage cultures.
Why the study?
African swine fever lacks vaccines, and the unknown functions and antigenic properties of many ASFV proteins impede vaccine development.
The ASFV proteins p285L and pK145R are non-essential for in vitro propagation of the virus, but their deletion may be useful for serological differentiation from wild-type-infected animals.
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p285L/pK145R non-essential for in vitro ASFV growth; leaves open DIVA vaccine utility pending in vivo testing.
Hübner et al. (2019) studied African swine fever. Deletion of 285L or K145R vs. Wild-type ASFV was evaluated on Viral spread and productive growth in vitro. Deletion of 285L or K145R from the ASFV genome did not significantly affect viral spread and productive growth in wild boar lung cells or primary macrophage cultures.