What question did this study set out to answer?

To evaluate the binding affinity, metal selectivity, and structural recognition mechanisms of a monoclonal antibody raised against cadmium-EDTA complexes.

November 1, 1996Journal of Biological ChemistryOpen Access

Metal Binding Properties of a Monoclonal Antibody Directed toward Metal-Chelate Complexes

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Authors

DBDiane A. BlakeTulane University PCPampa ChakrabartiMeharry Medical College MKMehraban KhosravianiBioanalytica (Switzerland)

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Implication

Laboratory study demonstrates selective nanomolar affinity of a monoclonal antibody for cadmium and mercury chelates, suggesting potential for targeted toxic metal sensing and remediation.

Key Points

To evaluate the binding affinity, metal selectivity, and structural recognition mechanisms of a monoclonal antibody raised against cadmium-EDTA complexes.
Generated monoclonal antibodies by immunizing BALB/c mice with a cadmium-EDTA complex covalently linked to a carrier protein.
Measured equilibrium dissociation constants across 16 distinct metal-EDTA complexes using a KinExA immunoassay instrument.
Deduced heavy- and light-chain variable region sequences using PCR amplification to construct three-dimensional molecular models of the binding site.
The antibody bound cadmium-EDTA and mercury-EDTA complexes with high affinity, exhibiting equilibrium dissociation constants of 21 nM and 26 nM, respectively.
Affinities for 14 other metal-EDTA complexes were 20- to 40,000-fold weaker and correlated with divalent metal ion radius, excluding mercury and magnesium.
Molecular modeling identified heavy chain histidine 96 as the essential residue facilitating coordinated cadmium recognition.

Cite This Study

Blake et al. (1996) studied this question.

synapsesocial.com/papers/6a9e54d4dfdaa94ae16aadf1 https://doi.org/10.1074/jbc.271.44.27677

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Implication

Laboratory study demonstrates selective nanomolar affinity of a monoclonal antibody for cadmium and mercury chelates, suggesting potential for targeted toxic metal sensing and remediation.

Metal Binding Properties of a Monoclonal Antibody Directed toward Metal-Chelate Complexes

Discussion

Member takes

Implication

Key Points

Cite This Study

Metal Binding Properties of a Monoclonal Antibody Directed toward Metal-Chelate Complexes

Discussion

Member takes

Implication

Key Points

Cite This Study

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