Key result
Polymerase chain reaction (PCR) detected bovine viral diarrhea virus in 77% (46/60) of positive tissue homogenates using one primer set, and in 10 of 11 remaining samples using a second set.
Observational (n=60)
No
PCR can effectively detect BVDV directly from tissue homogenates in a diagnostic setting.
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Supports PCR detection of BVDV from tissue homogenates in diagnostics; leaves open validation in larger field cohorts.
Tissue homogenates from 60 specimens submitted to the Veterinary Diagnostic Center were evaluated by polymerase chain reaction (PCR) for detection of bovine viral diarrhea virus (BVDV). Conventional virus isolation procedures showed the specimens contained BVDV. The BVDV RNA was extracted from the homogenates and subjected to a reverse transcription reaction followed by PCR amplification. The PCR product was blotted onto a nylon membrane and hybridized with a 30-base pair oligonucleotide probe labeled with 32P. One set of PCR primers detected BVDV in 46/60 (77%) of the tissue homogenates. An additional set of primers was used to detect 10/11 samples that had escaped detection with the first set of primers. The results indicate that BVDV can be detected by PCR directly out of tissue homogenates generated in a diagnostic setting.
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Schmitt et al. (1994) conducted an observational in Bovine viral diarrhea virus (BVDV) (n=60). Polymerase chain reaction (PCR) vs. Conventional virus isolation was evaluated on Detection of BVDV. Polymerase chain reaction (PCR) detected bovine viral diarrhea virus in 77% (46/60) of positive tissue homogenates using one primer set, and in 10 of 11 remaining samples using a second set.
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