What question did this study set out to answer?

To determine the role of brain-derived neurotrophic factor (BDNF) in the differentiation, proliferation, and long-term survival of EGF-responsive neural stem cell-derived precursors.

August 1, 1995Journal of NeuroscienceOpen Access

BDNF enhances the differentiation but not the survival of CNS stem cell- derived neuronal precursors

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Authors

SASohail AhmedAir University BRBA ReynoldsUniversity of Calgary SWSamuel WeissUniversity of Calgary

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Implication

In vitro study demonstrates that BDNF stimulates differentiation and neurite outgrowth in striatal neural precursors, indicating it does not sustain long-term neuronal survival alone.

Key Points

To determine the role of brain-derived neurotrophic factor (BDNF) in the differentiation, proliferation, and long-term survival of EGF-responsive neural stem cell-derived precursors.
Assessed Trk family neurotrophin receptor mRNA expression in undifferentiated mouse striatal precursor spheres using RT-PCR.
Treated EGF-generated precursor spheres with single, repeated, or delayed applications of BDNF and evaluated neuron counts, neurite outgrowth, and cell division markers (c-fos and BrdU) across 10, 21, and 27 days in vitro.
A single application of BDNF induced a twofold increase in neuron counts and promoted neurite outgrowth at 10 days in vitro without stimulating precursor cell proliferation.
The twofold increase in neuron number waned significantly by day 21 and was absent by day 27, an attrition that repeated BDNF dosing failed to prevent despite sustaining increased neurite numbers.
BDNF selectively expanded the population and neurite complexity of neurons co-expressing GABA and substance P rather than inducing a novel phenotype.

Cite This Study

Ahmed et al. (1995) studied this question.

synapsesocial.com/papers/6a9e600c74bfeee0946aae5b https://doi.org/10.1523/jneurosci.15-08-05765.1995

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Implication

In vitro study demonstrates that BDNF stimulates differentiation and neurite outgrowth in striatal neural precursors, indicating it does not sustain long-term neuronal survival alone.

BDNF enhances the differentiation but not the survival of CNS stem cell- derived neuronal precursors

Discussion

Member takes

Implication

Key Points

Cite This Study

BDNF enhances the differentiation but not the survival of CNS stem cell- derived neuronal precursors

Discussion

Member takes

Implication

Key Points

Cite This Study

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