Key result
PCR and sequence analysis of amplified VP1 cDNA from suspect cattle showed 78% homology with O1K and over 95% homology between samples, suggesting the 2 outbreaks shared the same virus serosubtype.
PCR and VP1 sequencing can effectively detect and subtype foot-and-mouth disease virus in infected cattle, aiding in outbreak investigation.
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May aid FMDV outbreak tracing in cattle; leaves open prospective validation before routine use.
Fast and accurate detection of foot-and-mouth disease (FMD) outbreaks is needed to limit spread of the disease by proper vaccination. The use of the polymerase chain reaction (PCR) has revolutionized the way in which viral diseases are diagnosed. Sequence analysis of the amplified VP1 sequence can enable the classification of FMD virus detected in the morbid animal. PCR assays were carried out to identify the virus and its serotype in suspect animals from 2 outbreaks of FMD type O virus. Sequence analysis of the amplified VP1 cDNA showed 78% homology with O1K and over 95% homology between the samples. These findings suggest that the 2 outbreaks were due to infection with the same virus serosubtype.
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Stram et al. (1995) studied Foot-and-mouth disease (FMD). Polymerase chain reaction (PCR) and amplified VP1 sequencing was evaluated on Virus identification and serotype classification. PCR and sequence analysis of amplified VP1 cDNA from suspect cattle showed 78% homology with O1K and over 95% homology between samples, suggesting the 2 outbreaks shared the same virus serosubtype.
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