Key result
Polyclonal and six monoclonal antibodies against FMDV VP1 were successfully generated and characterized, demonstrating utility for detecting serotypes A and O of FMDVs for diagnostic usage.
The generated monoclonal and polyclonal antibodies against VP1 are effective at detecting FMDV serotypes A and O, providing useful tools for early diagnostic applications.
Antibodies enable FMDV detection in preclinical models; leaves open validation before diagnostic adoption.
The foot and mouth disease virus (FMDV) is an RNA virus composed of single stranded positive sense RNA. FMDV has been known to infect cloven-hoofed animals, including pigs, cattle, and sheep. FMDV is rapidly spreading outward to neighboring regions, often leading to a high mortality rate. Thus, early diagnosis of FMDV is critical to suppress propagation of FMDV and minimize economic losses. In this study, we report the generation and characterization of polyclonal and six monoclonal antibodies against VP1 through immunoblotting and immunofluorescence microscopy analyses. These VP1 antibodies will be useful as tools to detect serotypes A and O of FMDVs for diagnostic usage.
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Cho et al. (2012) studied Foot and mouth disease virus (FMDV). Monoclonal and polyclonal antibodies against FMDV VP1 was evaluated on Antibody generation and characterization. Polyclonal and six monoclonal antibodies against FMDV VP1 were successfully generated and characterized, demonstrating utility for detecting serotypes A and O of FMDVs for diagnostic usage.
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