Key result
The MELISA assay specifically detected FMDV serotype O with a detection limit of 0.04 ng, demonstrating higher sensitivity than traditional ELISA without cross-reactivity to other serotypes.
Observational (n=125)
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An ELISA assay with monoclonal antibody (MELISA) was used to type serotype O of foot-and-mouth disease virus (FMDV). All FMDV serotype O reference strains were positive by MELISA, while other viruses such as FMDV serotypes Asia 1, C, and A and classical swine fever virus, swine vesicular disease virus, and porcine reproductive and respiratory syndrome virus remained negative. Furthermore, FMDV serotype O positive samples were able to be detected by MELISA. This assay may be particularly suitable for diagnosis of FMDV serotype O infection in field stations.
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Offers a more sensitive tool for FMDV serotype O detection; leaves open field validation before diagnostic adoption.
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Chen et al. (2013) conducted an observational in Foot-and-Mouth Disease Virus (FMDV) serotype O infection (n=125). Monoclonal antibody-based ELISA (MELISA) vs. Traditional ELISA was evaluated on Detection limit for purified viruses (ng). The MELISA assay specifically detected FMDV serotype O with a detection limit of 0.04 ng, demonstrating higher sensitivity than traditional ELISA without cross-reactivity to other serotypes.
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