Key result
The MELISA assay was highly specific and four times more sensitive than traditional ELISA for detecting FMDV serotype O, successfully identifying all 32 positive clinical samples without cross-reactivity.
Population
125 clinical samples from infected pigs and reference strains of FMDV and other viruses
Comparison
ELISA assay with monoclonal antibody (MELISA) vs Traditional ELISA
Design
Preclinical
Authors
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May improve FMDV O detection in samples; leaves open clinical adoption pending prospective validation.
The MELISA assay provides a highly sensitive and specific method for rapid detection and typing of FMDV serotype O in clinical samples.
Chen et al. (2012) studied Foot-and-mouth disease virus (FMDV) serotype O infection (n=125). MELISA (ELISA assay with monoclonal antibody) vs. Traditional ELISA was evaluated on Detection of FMDV serotype O. The MELISA assay was highly specific and four times more sensitive than traditional ELISA for detecting FMDV serotype O, successfully identifying all 32 positive clinical samples without cross-reactivity.
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