Key result
Automated patch-clamp techniques yielded IC50 values for Cav3.1 T-type calcium channel blockers that correlated well with conventional patch-clamp methods (R = 0.82), demonstrating its reliability for drug screening.
Automated patch-clamp is an efficient and reliable method for ranking drug potencies for T-type calcium channels.
Supports automated screening of T-type channel blockers; extends method validation yet leaves open in vivo and clinical translation.
T-type calcium channels are involved in a variety of physiological and pathophysiological processes, and thus could be therapeutic targets. However, there is no T-type channel selective blocker for use in clinical practice, demanding a need for the development of novel drugs where a higher-throughput screening system is required. Here we present pharmacological studies on Ca(v)3.1 T-type channels using automated patch-clamp. The IC(50) values obtained from automated patch-clamp and conventional one showed a good correlation (correlation coefficient of 0.82), suggesting that the automated patch-clamp is an efficient and reliable method for ranking the drug potencies for T-type channels.
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Choi et al. (2011) studied this question. Automated patch-clamp technique vs. Conventional patch-clamp technique was evaluated on Correlation of IC50 values between automated and conventional patch-clamp (R = 0.82). Automated patch-clamp techniques yielded IC50 values for Cav3.1 T-type calcium channel blockers that correlated well with conventional patch-clamp methods (R = 0.82), demonstrating its reliability for drug screening.
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