Real‐Time Monitoring of DNA Polymerase Function and Stepwise Single‐Nucleotide DNA Strand Translocation through a Protein Nanopore

Stepping it forward: A major challenge in realizing single-molecule nanopore DNA sequencing is to devise a way to ratchet DNA strands through a nanopore at appropriate speed that is commensurate with real-time sequential nucleobase identification. A promising approach is presented that exploits the intrinsic motor function of DNA polymerases to effect DNA strand translocation through an α-hemolysin nanopore (see picture).