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High-performance liquid-chromatographic separation of bile acids, free and conjugated with taurine and glycine, is described. The analysis of free and glycine conjugated bile acids is based on the esterification of the carboxylic group of bile acids with 1-p-nitrobenzyl-3-p-tolyltriazene. On the other hand, taurine conjugated bile acids are separated and detected by an ultraviolet detector (210 nm), directly.
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Okuyama et al. (1976) studied this question.
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