January 1, 1981European Journal of Biochemistry
The Amino Acid Sequence of the δ Subunit (Calmodulin) of Rabbit Skeletal Muscle Phosphorylase Kinase
Key result
The amino acid sequence of the phosphorylase kinase delta subunit was determined to contain 148 amino acids and is identical to bovine uterus calmodulin.
Population
Rabbit skeletal muscle phosphorylase kinase delta subunit (calmodulin)
Design
Preclinical
Authors
Discussion
Loading...
Member takes
Implication
Identifies delta subunit as calmodulin in rabbit muscle; extends Ca2+ regulation insights but leaves open human cardiac translation.
Structured PICO
P
Population
Rabbit skeletal muscle phosphorylase kinase delta subunit (calmodulin)
E
Exposure
Amino acid sequencing using cyanogen bromide peptides and tryptic peptides
O
Outcome
Amino acid sequence determination
The study determined the amino acid sequence of the phosphorylase kinase delta subunit from rabbit skeletal muscle, identifying it as identical to bovine uterus calmodulin.
Limitations
- The sequence of the N-terminal tripeptide and the amide assignments on residues 58 and 60 were not determined unequivocally.
Cite This Study
Grand et al. (1981) studied this question. The amino acid sequence of the phosphorylase kinase delta subunit was determined to contain 148 amino acids and is identical to bovine uterus calmodulin.