Key result
Three-dimensional imaging technologies, including TEM, electron tomography, and FIB-SEM, are providing deeper insights into the structural and dynamic aspects of viral infection.
3D imaging technologies, including TEM, electron tomography, and FIB-SEM, are crucial for understanding the structural and dynamic aspects of viral infections in cells.
Advances viral imaging insights; leaves open clinical translation in virology and cardiology.
Three-dimensional (3D) imaging technologies are beginning to have significant impact in the field of virology, as they are helping us understand how viruses take control of cells. In this article we review several methodologies for 3D imaging of cells and show how these technologies are contributing to the study of viral infections and the characterization of specialized structures formed in virus-infected cells. We include 3D reconstruction by transmission electron microscopy (TEM) using serial sections, electron tomography, and focused ion beam scanning electron microscopy (FIB-SEM). We summarize from these methods selected contributions to our understanding of viral entry, replication, morphogenesis, egress and propagation, and changes in the spatial architecture of virus-infected cells. In combination with live-cell imaging, correlative microscopy, and new techniques for molecular mapping in situ, the availability of these methods for 3D imaging is expected to provide deeper insights into understanding the structural and dynamic aspects of viral infection.
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Risco et al. (2014) conducted a review in Viral infections. Three-dimensional (3D) imaging technologies was evaluated. Three-dimensional imaging technologies, including TEM, electron tomography, and FIB-SEM, are providing deeper insights into the structural and dynamic aspects of viral infection.
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