Thirty-eight female water buffalo calves were divided into four groups. In Group 1 (control) each calf received 340 kg of milk in 120 days. In Groups 2, 3, and 4 each calf was fed on 103 kg of milk in 45 days, plus a high quality ration which was gradually replaced by a low quality ration after 45 days, 75 days, and 120 days, respectively. Half of the calves in each group were inoculated with the rumen contents obtained from a fistulated adult buffalo. The establishment of rumen ciliate protozoa, microbial activity, and growth
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Borhami et al. (1967) studied this question.
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