Key result
In ovine granulosa cells, ANP significantly reduced oestradiol and increased progesterone (P<0.05), and both ANP and CNP inhibited VEGF164 expression (P<0.01) after 48 hours of culture.
Why the study?
Although natriuretic peptides have critical roles in rodent follicular development and oocyte maturation, their signalling pathways and mechanisms of action in monovulatory species follicles required further understanding.
p-value: p=<0.05
Natriuretic peptide responsiveness in sheep follicle granulosa cells is multi-facilitated, utilizing both autocrine and paracrine stimulation pathways to regulate steroidogenesis and angiogenesis markers.
Caution against human translation from ovine cells; leaves open natriuretic peptide roles in ovarian steroidogenesis and angiogenesis.
Natriuretic peptides (NPs) have been reported to have critical roles in follicular development and oocyte maturation in rodents. This study aimed to extend our current understanding of NP-mediated signalling pathways and mechanisms of action in the follicles of a monovulatory species. Ovine granulosa cells (GCs) and theca cells (TCs) were cultured under conditions designed to allow gonadotrophin-stimulated cell differentiation. Gene expression analysis was performed by qualitative (q)PCR for NPs and NPRs (between 16 and 96 h of culture) and VEGF120 and VEGF164 (between 16 and 144 h of culture). A qualitative analysis of the production of NP/NPR family members and NP ligand/receptor associations was carried out utilising a highly sensitive immunological approach known as 'proximity ligation assay' (PLA). All NPRs were observed in GCs, while NPRA was absent in TCs. In GCs, gene expression of NPRA, NPRB and NPRC was apparent but only active BNP and CNP and not ANP, were detected. Also in GCs, ANP but not CNP was able to significantly (P < 0.05) reduce oestradiol and increase (P < 0.05) progesterone. Inhibition of VEGF164 by ANP and CNP (P < 0.01) after 48 h of culture preceded up-regulation of VEGF120 by ANP (P < 0.01) after 144 h, but not CNP. Taken together, these findings appear to demonstrate that NP responsiveness in the GC compartment of sheep follicles is multi-facilitated, utilising both autocrine and paracrine stimulation pathways.
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Al‐Hamdan et al. (2020) studied Follicular development and oocyte maturation. Natriuretic peptides (ANP, BNP, CNP) was evaluated on Gene expression of NPs, NPRs, VEGF120, VEGF164, and hormone production (p=<0.05). In ovine granulosa cells, ANP significantly reduced oestradiol and increased progesterone (P<0.05), and both ANP and CNP inhibited VEGF164 expression (P<0.01) after 48 hours of culture.
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