Answers are grounded in this paper and cite their sources.
The effect of temperature and primer concentration on oligonucleotide-primed transcription has been studied using the separated strands of a well-defined natural DNA as template. Results were similar to those obtained in the homopolymer-directed model systems. At high temperature and excess primer concentration mainly primer-initiated RNA copies are synthesized. Omission of one ribonucleoside triphosphate also makes the termination specific. The unique RNA fragments thus obtained have been used to determine the perfectly-repeated sequence of 68 base pairs in this DNA.
No takes yet. Share an insight, caveat, or question.
Kreijl et al. (1977) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: