A miniaturized cell based on electrochemiluminescence of tris(2, 2′-bipyridine)ruthenium (II) complex was prepared to determine peptides in small volume of 0.5cm3. A calibration for proline gave a straight line in the concentration range 1.0×10-5∼1.0×10-3M (correlation coefficient: 0.991). Stronger responses for proline and histidine than other amino acids prompted us to measure some peptides. Out of six peptides, PG having proline at the amino terminal showed the strongest response.
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Egashira et al. (2000) studied this question.
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