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We describe a method to harden the nucleus of crystalline lenses in postmortem human globes by intralenticular injection of a mixture of paraformaldehyde and glutaraldehyde (Karnovsky's solution). Evaluation of the nuclear manipulations, using the Miyake-Apple posterior video/photographic technique, shows that the method provides suitable specimens for practicing extracapsular cataract extraction and phacoemulsification.
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Pandey et al. (2000) studied this question.
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