Key result
Chlorogenic acid effectively inhibited Enterovirus 71 replication in vitro with an IC50 of /ml and reduced the secretion of inflammatory cytokines.
Why the study?
Does chlorogenic acid inhibit Enterovirus 71 replication in vitro?
Does chlorogenic acid inhibit Enterovirus 71 replication in vitro?
Chlorogenic acid demonstrates in vitro antiviral properties against Enterovirus 71 by inhibiting viral replication and reducing pro-inflammatory cytokine secretion.
Answers are grounded in this paper and cite their sources.
In vitro EV71 inhibition by chlorogenic acid is hypothesis-generating; leaves open any therapeutic role pending in vivo validation.
Enterovirus 71 (EV71) is an etiology for a number of diseases in humans. Traditional Chinese herbs have been reported to be effective for treating EV71 infection. However, there is no report about the antiviral effects of CHA against EV71. In this study, plaque reduction assay demonstrated that the inhibitory concentration 50% (IC50) of CHA on EV71 replication is 6.3 µg/ml. When both CHA (20 µg/ml) and EV71 were added, or added post-infection at different time points, CHA was able to effectively inhibit EV71 replication between 0 and 10 h. In addition, CHA inhibited EV71 2A transcription and translation in EV71-infected RD cells, but did not affect VP1, 3C, and 3D expression. Furthermore, CHA inhibited secretions of IL-6, TNF-α, IFN-γ and MCP-1 in EV71-infected RD cells. Altogether, these results revealed that CHA may have antiviral properties for treating EV71 infection.
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Li et al. (2013) studied Enterovirus 71 (EV71) infection. Chlorogenic Acid vs. DMSO control was evaluated on Inhibition of EV71 replication (IC50) (IC50 6.3 µg/ml). Chlorogenic acid effectively inhibited Enterovirus 71 replication in vitro with an IC50 of /ml and reduced the secretion of inflammatory cytokines.
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