Key result
VSV-mp and VSV-sp clones isolated from persistently infected L cells were more temperature sensitive, grew slower, and induced interferon production compared to the parent VSV-o virus.
Virus clones isolated from persistently infected L cells exhibit altered growth properties and increased interferon induction and sensitivity compared to the parent virus.
These cell-culture adaptations in persistent VSV infection leave open relevance to in vivo pathogenesis or therapy; further animal studies required.
Two virus clones, VSV-mp and VSV-sp, were isolated from L cells persistently infected with VSV (New Jersey serotype). Both clones were more temperature sensitive than the parent virus, VSV-o, and grew more slowly, gave smaller plaques, less c.p.e. and lower virus yields in L cells. Unlike the parent virus, both persistent viruses induced interferon production in L cells. Stable carrier cultures could be obtained from L cells infected with VSV-sp at low multiplicities without pretreatment with interferon. This may be related to the fact that VSV-sp is more sensitive to interferon than either VSV-mp or VSV-o.
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Nishiyama et al. (1978) studied Persistent Infection of L Cells with VSV. VSV-mp and VSV-sp clones vs. Parent virus, VSV-o was evaluated on Viral properties (temperature sensitivity, growth rate, plaque size, cytopathic effect, virus yields, interferon production). VSV-mp and VSV-sp clones isolated from persistently infected L cells were more temperature sensitive, grew slower, and induced interferon production compared to the parent VSV-o virus.
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