Key result
Single amino acid substitutions in intact staphylococcal nuclease proteins can fail to stimulate T cell clones even when the same substitutions in synthetic peptides are stimulatory.
Amino acid substitutions in synthetic peptides may not accurately reflect their effects on T cell recognition in the intact protein due to differences in antigen processing.
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Caution advised extrapolating synthetic peptide data to native protein immunogenicity; leaves open antigen processing effects in T cell epitope studies.
Finnegan et al. (1992) studied this question. Single amino acid substitutions in intact staphylococcal nuclease protein vs. Synthetic peptides with corresponding substitutions and native Nase protein was evaluated on T cell clone stimulation/reactivity. Single amino acid substitutions in intact staphylococcal nuclease proteins can fail to stimulate T cell clones even when the same substitutions in synthetic peptides are stimulatory.
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