Systematic procedure for the fractionation of muscle protein, with particular reference to biochemical evaluation of meat quality.

A systematic scheme has been developed by improving Helander's method for the separation of principal intra-cellular proteins from muscle. Helander's technique with certain modifications has been integrated with other procedures, used for the fractionation of extracellular protein (stroma) and its biophysical characterization, to make the scheme more befitting for biochemical evaluation of meat quality. Besides simplicity, reproducibility and accuracy, this scheme has many inherent advantages over the previous methods, employed for this purpose. The design of an economical extraction apparatus, which helps minimizing the experimental error in handling the samples is also presented. Any number of samples can be extracted per unit time by making a suitable assembly of the design.