Key result
Type V collagen coating reduced both unstimulated and ADP-induced platelet adherence compared to uncoated plastic surfaces and Types I, III, and IV collagen, suggesting it is less thrombogenic.
Why the study?
Does Type V collagen coating reduce platelet adherence compared to other collagen types or uncoated surfaces in human platelet-rich plasma?
Does Type V collagen coating reduce platelet adherence compared to other collagen types or uncoated surfaces in human platelet-rich plasma?
Type V collagen appears to be less thrombogenic than Types I, III, or IV, as it reduces platelet adherence in vitro.
May support testing Type V collagen coatings in vascular devices; leaves open translation to human platelet responses.
Different types of collagen vary in their influence on platelet reactivity. Collagen Types III, IV, and V were obtained from human placental tissue, and Type I collagen was prepared from rat skin. Each collagen type was coated onto a plastic surface. Each collagen-coated surface or appropriate plastic surface control was studied using citrated human 51Cr-labeled platelet-rich plasma in both the presence and absence of 10 microM adenosine 5'-diphosphate (ADP). Both unstimulated and ADP-induced platelet adherence were: 1) reduced by Type V collagen coating in comparison to uncoated wells; and 2) increased by Types III and IV collagen coating in comparison to Type V coated or plastic surfaces. Addition of the fast-acting thrombin inhibitor dansylarginine (DAPA) had no significant effect on unstimulated and ADP-induced platelet adherence to Type III, IV or V collagen-coated surfaces. Type I collagen-coated surfaces, studied only in the presence of DAPA, caused greater platelet adherence than those coated with Types III, IV, or V collagen. We conclude that Type V collagen may be less thrombogenic than Types, I, III, or IV.
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Parsons et al. (1983) studied this question. Type V collagen coating vs. Uncoated plastic surfaces and Types I, III, and IV collagen was evaluated on Platelet adherence (unstimulated and ADP-induced). Type V collagen coating reduced both unstimulated and ADP-induced platelet adherence compared to uncoated plastic surfaces and Types I, III, and IV collagen, suggesting it is less thrombogenic.
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