Key result
Transfection of a full-length viral clone of TTV genotype 6 generated at least six proteins, with two localizing primarily to the nucleus, two to the cytoplasm, and two throughout the cell.
The TTV genotype 6 genome generates at least six proteins with distinct cellular localizations following transfection.
TTV genotype 6 protein localization patterns warrant caution in inferring in vivo roles; leaves open functional significance in human infection.
The expression profile of the circovirus TTV has not yet been fully characterized. In this paper, we show that following transfection of a full-length viral clone of TTV genotype 6, each of the three virally encoded mRNAs is translated from two initiating AUGs, and therefore, the TTV genome generates at least six proteins. Localization studies of hemagglutinin-tagged versions of these proteins in fixed cells, and green fluorescent protein-tagged versions of these proteins in living cells, expressed following transfection, demonstrated that two were primarily nuclear, two were primarily cytoplasmic, and two were found throughout the cell.
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Qiu et al. (2005) studied Circovirus TTV genotype 6. Transfection of a full-length viral clone of TTV genotype 6 was evaluated on Protein expression and localization. Transfection of a full-length viral clone of TTV genotype 6 generated at least six proteins, with two localizing primarily to the nucleus, two to the cytoplasm, and two throughout the cell.
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